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A cytogenetically anchored genetic map of bovine chromosome 1 obtained by integrating flow-sorted chromosome-derived microsatellite markers into the international bovine map

Identifieur interne : 00D102 ( Main/Exploration ); précédent : 00D101; suivant : 00D103

A cytogenetically anchored genetic map of bovine chromosome 1 obtained by integrating flow-sorted chromosome-derived microsatellite markers into the international bovine map

Auteurs : D. Vaiman [France] ; L. Schibler [France] ; A. Oustry [France] ; A. Schmitz [France] ; J.-P. Furet [France] ; W. Barendse [Australie] ; E. P. Cribiu [France]

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RBID : Pascal:98-0243697

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English descriptors

Abstract

A genomic library was constructed from a peak of flow-sorted bovine chromosomes 1 + X after PCR amplification. Forty-three bovine chromosome I microsatellites were isolated, genetically mapped and integrated in the international genetic map. In addition, BAC clones from a goat BAC library were identified for five markers (DVEPC119, INRA011, BM4307, KAP8 and MAF64). These goat BACs could be mapped by FISH onto bovine chromosome I to bands 1q44→q45, 1q25, 1q21, 1q12and 1q14→q21, respectively. This map reduces the average interval between consecutive markers on the international bovine genetic map from 5.5 cM to 2.5 cM, and provides a good starting point for positional cloning projects in cattle, sheep or goats.


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Le document en format XML

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<div type="abstract" xml:lang="en">A genomic library was constructed from a peak of flow-sorted bovine chromosomes 1 + X after PCR amplification. Forty-three bovine chromosome I microsatellites were isolated, genetically mapped and integrated in the international genetic map. In addition, BAC clones from a goat BAC library were identified for five markers (DVEPC119, INRA011, BM4307, KAP8 and MAF64). These goat BACs could be mapped by FISH onto bovine chromosome I to bands 1q44→q45, 1q25, 1q21, 1q12and 1q14→q21, respectively. This map reduces the average interval between consecutive markers on the international bovine genetic map from 5.5 cM to 2.5 cM, and provides a good starting point for positional cloning projects in cattle, sheep or goats.</div>
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